This book titled, ‘Practical Handbook: Instrumental Methods of Analysis’ covers all the experiments prescribed in the course of ‘Instrumental Methods of Analysis’ of 7th semester, B.Pharmacy and also the common practicals prescribed in the syllabus of all branches of 1st semester, M.Pharmacy. Sincere effort has been made to present the reader with clear, succinct and easy to understand practical experiments.

   The content of this book will be beneficial for students, researchers and faculty members. Appendices provide the detailed list of experimental conditions required by drugs and pharmaceutical products which are assayed by UV-Visible Spectroscopy, Fluorimetry, Flame Photometry, Nephelo-turbidometry, Column Chromatography, Thin Layer Chromatography and HPLC.

 1. To determine the λmax of Phenol and effect of solvents on absorption spectra of phenol.
 2. To determine the concentration of dextrose in given unknown sample by colorimetry.
 3. To estimate amount of Sulfanilamide by colorimetry.
 4. To carry out assay of Paracetamol tablets I.P. by UV Spectrophotometry.
 5. To determine the percentage purity of Furosemide tablet by UV  Spectrophotometry.
 6. To determine the percentage purity of Chloramphenicol capsules by UV Spectrophotometry.
 7. To determine the percentage purity of Ampicillin injection by single point standerdisation method using UV-Visible Spectrophotometer.
 8. To determine the concentration of Ibuprofen and Paracetamol present in a combined formulation by using simultaneous equation method.
 9. To determine the concentration of Ofloxacin and Ornidazole present in a combined formulation by using simultaneous equation method.
10. To determine the concentration of Ibuprofen and Paracetamol in an unknown mixture by using absorption ratio (Q analysis) method.
11. To determine the concentration of Aspirin in Dextropropoxyphen compound capsules by using difference spectrophotometric method.
12. To determine the concentration of quinine sulfate in given tablet by fluorimetry.
13. To perform the assay of Riboflavin tablets by fluorimetry.
14. To study the effect concentration on fluorescence intensity of quinine sulphate solution.
      (To study the self quenching behaviour of quinine sulphate solution)
15. To study the “Quenching” effect due to the presence of varying concentration of iodide ions on the fluorescence of quinine sulphate solution.
16. To determine the concentration of Sodium in given sample by Flame Photometry. >
17. To determine the concentration of Potassium in given unknown sample by Flame Photometry.
18. To determine sulfate ion concentration in a water sample by Nephelo-Turbidometry.
19. To determine phosphate ion concentration in a water sample by Nephelo-Turbidometry
20. To separate and identify the given mixture of Amino acid by ascending paper chromatography.
21. To separate and identify the given mixture of Amino acid by desending paper chromatography
22. To identify the given unknown mixture of Amino acid by circular paper chromatography.
23. To separate and identify the given mixture of sugar by thin layer chromatography.
24. To separate plant pigments from spinach leaves using column chromatography.
25. To perform the assay of Paracetamol in given tablets using calibration curve methods by HPLC.
26. To perform the assay of Paracetamol in given tablets using single point standardization methods by HPLC.
27. To determine caffeine and benzoic acid in given sample (soft drink) by HPLC.
28. To perform the assay of methyltestosterone tablet by GC.

   APPENDIX – I
      Table 1. UV-Visible absorption characteristics of pharmaceutical substances.
      Table 2. Visible absorption characteristics of pharmaceutical substances. (Colorimetric determination)
      Table 3. Determination of fluorescent pharmaceutical substances by fluorimetry.
      Table 4. Determination of non fluorescent pharmaceutical substances by chemical reaction (fluorimetry)
      Table 5. Assay of pharmaceutical substances by flame emission spectrometry.
      Table 6. Assay of antibiotics by nepehlo-turbidimetry
     Table 7. Determination of ions by nepehlo-turbidimetry
     Table 8. Determination of inorganic substances by nephlo-turbidimetry

  Appendix – II
     Table 1. Eluotropic series of solvents used in chromatography
     Table 1. Eluotropic series of solvents used in chromatography
     Table 2. Detection of impurity in drugs by TLC
     Table 3. Detection of degradation product by TLC
     Table 4. Detection of related compounds in drugs by TLC
     Table 5. Determination of pharmaceutical substances by HPLC